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. 2021 Oct 15;12:713400. doi: 10.3389/fgene.2021.713400

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Copyright © 2021 Fijalkowski, Peeters and Van Damme.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Reverse-Phase High-Performance Liquid Chromatography (RP-HPLC) investigation of peptide solubilization biases in selected protocols under study. RP-HPLC chromatograms of a synthethic peptide mix processed with the 5 experimental procedures described in this study (graph headers). Arbitrary units of absorption at 280 nm are presented across the 100 min ascending ACN gradient applied. The expected elution interval of missing peptides are indicated with an asteriks. A representative replicate is shown for each setup.