Skip to main content
. 2021 Oct 15;12:754421. doi: 10.3389/fgene.2021.754421

FIGURE 6.

FIGURE 6

LCN2 and RAB27B might act as biomarkers in the meniscus for early osteoarthritis (OA) diagnosis. (A) Relative expression levels of LCN2, RAB27B, PRDM1, and SERPINB2 in IL-1β-treated menisci. GAPDH was used as the internal reference gene for qRT-PCR relative expression. Error bars reveal the standard deviation or the standard error of the data. The statistical methods are described above. *p < 0.05, **p < 0.01, ***p < 0.001. (B) Relative expression levels of LCN2 and RAB27B in menisci treated with time-dependent IL-1β stimulation (0, 12, 24, 48, 72, and 96 h). GAPDH was used as the internal reference gene for qRT-PCR relative expression. Error bars reveal the standard deviation or the standard error of the data. (C) Safranin O/Fast Green staining of patients’ knee cartilage and immunohistochemical (IHC) staining of patients’ menisci with antibody against LCN2 and RAB27B. (D) Correlation coefficient between LCN2 and RAB27B expression quantified by IHC positive cell percentage and OARSI score in patients’ samples (n = 5). (E) Safranin O and IHC staining of LCN2 and RAB27B in mice knee at 1, 2, 4, 8, and 26 weeks and quantification of positive cells (n = 3). Error bars reveal the standard deviation or the standard error of the data. Scale bar, 50 μm.