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. 2021 Oct 15;12:750489. doi: 10.3389/fmicb.2021.750489

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Copyright © 2021 Boudet, Sorlin, Pouget, Chiron, Lavigne, Dunyach-Remy and Marchandin.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Dynamics of biofilm formation in BHI medium and ASM in the absence of antibiotics for two strains isolated 3years apart in Patient 17 (strain 17.2: “early” strain and 17.6: “late” strain). Left: Biofilm formation after 5h of incubation: (A,B) early strain 17.2 in BHI medium (A) and ASM (B); (C,D) late strain 17.6 in BHI medium (C) and ASM (D). Right. Percentage of biofilm formed over time (in hours). p-values are indicated for comparisons of biofilm formed by a strain depending on the culture medium at each time of the assay. ^*Value of p<0.05 using t test.