Figure 5.

Dynamics of biofilm formation (% of biofilm formed) in BHI medium and ASM in the presence of antibiotics for two strains isolated 3years apart in Patient 17 (strain 17.2: “early” strain and 17.6: “late” strain). Results are presented comparatively to those observed in the absence of antibiotic previously presented in Figure 4. (A) After 5h of incubation; (B) after 12h of incubation; (C) after 36h of incubation. No, no antibiotic; LIN, linezolid; CTO, ceftobiprole; and CTL, ceftaroline. Antibiotics were added to a final concentration equivalent to the minimum biofilm inhibitory concentration (bMIC) in the corresponding medium as follows: strain 17.2, bMICs in BHI medium and in ASM: CTL: 0.125mg/L, CTO: 0.25mg/L, LIN: 0.5mg/L; strain 17.6, bMICs in BHI medium: CTL: 0.25mg/L, CTO: 0.5mg/L, LIN: 2mg/L; bMICs in ASM: CTL: 4mg/L, CTO: 4mg/L, and LIN: 2mg/L. Data are commented for statistical significance in the text.