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Primers used in this work.
| Primer | Sequence (5'–3')* | References |
|---|---|---|
| asnB_NcoI | GCATCCATGGGATGTGGATTTGTAGGATGCGTAC | This work |
| asnB_SalI | CACTGTCGACTTATTTTCCAAAATCGTATTTATCTGC | This work |
| pIMK_REV | CCTATCACCTCAAATGGTTCG | Rogiers et al., 2017 |
| pIMK_FW | GAGTCAGTGAGCGAGGAAGC | Rogiers et al., 2017 |
| NC16 | GTCAAAACATACGCTCTTATC | Rogiers et al., 2017 |
| Ylinker | CTGCTCGAATTCAAGCTTCT | Rogiers et al., 2017 |
| Marq269 | GCTCTGATAAATATGAACATGATGAGTGAT | Rogiers et al., 2017 |
Restriction sites: NcoI (CCATGG) and SalI (GTCGAC).
