Age-related changes in relative liver weight (A), and serum ALT (B) and AST (C) activities of laying hens. Data are presented as mean with the pooled standard error of each experimental group (8 replicates). abDifferent letters above the error bar indicate significant difference (P < 0.05). Abbreviations: ALT, alanine aminotransferase; AST, aspartate aminotransferase; D195, 195-day-old laying hens; D340, 340-day-old laying hens; D525, 525-day-old laying hens.