Figure 1.

Effect of garlic on protein expression of HMGCR, ABCA1 and CYP46A1 in mouse brain tissue. Mice were randomly allocated into 2 groups of 15 mice each and were administered with dH₂O or 150 mg/kg/weight aqueous extract of garlic, three times a week by oral gavage, for six weeks. At the end of the experiment, the mice were anesthetized and the brain was dissected, frozen immediately in liquid nitrogen, and stored in −70°C. Protein expression pattern of (A) HMG-CoA reductase, (B) ABCA1 (because of abnormal and the huge difference between ABCA1 band related to the third sample in the control group, it was excluded when plotting the quantitative data of imageJ presented in Figure 1B), and (C) CYP46A1 in the brain of control and garlic group was analyzed by western blotting. Equal loading was assessed with an anti β-actin antibody. Bar graph represents quantification of Western blots after normalizing against β-actin for a) HMGCAR, b) ABCA1, and c) CYP46A1. The mean±SEM of three independent experiments is shown. ^*p<0.05 and ^**p<0.01 present significant differences from the control group