Figure 2.

Effect of allicin on protein expression of HMGCR, ABCA1 and CYP46A1 in astrocytes isolated from C57BL/6J. Astrocytes were isolated and cultured in low-glucose DMEM containing 10% fetal bovine serum and 1% penicillin/streptomycin for a 1-week primary and a subsequent one-week secondary culture. Astrocytes were treated with 5 µg/ml allicin and after incubation time, cells were harvested and analyzed by western blotting by using specific antibody against (A) HMG-CoA reductase, (B) ABCA1, and (C) CYP46A1. Equal loading was assessed with an anti β-actin antibody. Bar graph represents quantification of Western blots after normalizing against GAPDH for a) HMGCAR, b) ABCA1, and c) CYP46A1. The mean±SEM of three independent experiments is shown. ^*p<0.05, ^**p<0.01, and ^***p<0.005 present significant differences from the control group