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. 2021 Oct 15;12:748706. doi: 10.3389/fmicb.2021.748706

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Copyright © 2021 Pavkova, Klimentova, Bavlovic, Horcickova, Kubelkova, Vlcak, Raabova, Filimonenko, Ballek and Stulik.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Profiles of cytokine secretion by bone marrow-derived macrophages (BMDM) stimulated with Francisella tularensis-derived outer membrane vesicles (Ft-OMV). BMDM were treated with two different doses of Ft-OMV or infected with F. tularensis FSC200. Four and 24 h after these stimulations, the cell supernatants were collected and the indicated cytokines were quantified by fluorescence-based multiplex microarray chip ELISA. Data are representative of two to four independent experiments. **p < 0.01, ***p < 0.001, ****p < 0.0001; two-way ANOVA with Tukey’s multiple comparisons test.