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. 2021 Oct 21;27(39):6615–6630. doi: 10.3748/wjg.v27.i39.6615

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©The Author(s) 2021. Published by Baishideng Publishing Group Inc. All rights reserved.

This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/Licenses/by-nc/4.0/

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Collagen fibers were automatically extracted for analysis using open-source software CT-FIRE. A: Histograms were generated to show the distribution of various parameters in each polarized light microscopy imaging; B: Quantitative analysis of collagen fibers from polarized light microscopy imaging in the colonic mucosa of different treatment groups. Data are mean ± SE of three images per tissues region. ^aP < 0.05, ^bP < 0.01, control compared with 1,2-dimethylhyrazine (DMH); ^cP < 0.05, ^dP < 0.01, DMH compared with cold exposure and capsaicin-treated group; ^eP < 0.05, Cold exposure compared with capsaicin-treated group.