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. 2021 Oct 21;2021:9577874. doi: 10.1155/2021/9577874

Figure 4.

Figure 4

Effect of the Nrf2 inhibitor on pain hypersensitivity and the spinal expression of Nrf2 in the nuclear extracts. (a, b) The analgesic effect of RTA-408 (10 μg, i.t.) in CCI mice was completely inhibited by the Nrf2 inhibitor trigonelline (Trig). Two-way ANOVA with repeated measures was performed, followed by the Bonferroni post hoc test (∗P < 0.05, ∗∗P < 0.01, and ∗∗∗P < 0.001 compared with the sham+vehicle group, #P < 0.05, ##P < 0.01, and ###P < 0.001 compared with the CCI+RTA+Trig group, n = 5 per group). (c) The spinal expression of Nrf2 in the nuclear extracts was significantly increased following CCI. Representative blots and blot density (normalized to histone H3 loading control) are presented (∗∗∗P < 0.001 compared with the sham+vehicle group). RTA-408 administration further increased the spinal expression of Nrf2 in the nuclear extracts in the CCI group (∗∗∗P < 0.001 compared with the CCI+vehicle group, n = 5 in each group), which was significantly inhibited by the preinjection of trigonelline. One-way ANOVA followed by Bonferroni analysis was used to test the differences among groups (∗∗∗P < 0.001 compared with the CCI+RTA-408 group, n = 5 per group).