Figure 8.

Effect of RTA-408 on mitochondrial protein and mtDNA content and on the spinal cord following CCI. The spinal cord was extracted and analyzed for (a) mitochondrial protein expression of PGC-1α, NRF1, and TFAM and (b) mtDNA content. The intensity of protein blots was normalized to a loading control GAPDH antibody and expressed as the fold of control. One-way ANOVA followed by Bonferroni analysis was used to test the differences among groups (^∗P < 0.05, ^∗∗P < 0.01, and ^∗∗∗P < 0.001 compared with the indicated group, n = 5 per group).