Figure 9.

Effect of trigonelline on mitochondrial protein and mtDNA in the spinal cord following CCI. The spinal cord was extracted and analyzed for (a) mitochondrial protein expression of PGC-1α, NRF1, and TFAM and (b) mtDNA content. The intensity of protein blots was normalized to a loading control GAPDH antibody and expressed as the fold of control. One-way ANOVA followed by Bonferroni analysis was used to test the differences among groups (^∗∗P < 0.01, ^∗∗∗P < 0.001 compared with the indicated group, n = 5 per group).