REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
anti-Yap(H-9)	Santa Cruz Biotechnology	Cat#sc-271134; RRID: AB_10612397
anti-Phospho-YAP (Ser127)	Cell Signaling Technology	Cat#13008; RRID: AB_2650553
anti-Sox9 (D8G8H)	Abcam	Cat#ab185966; RRID: AB_2728660
anti-Ki-67(D3B5)	Cell Signaling Technology	Cat#12075; RRID: AB_2728830
anti-Kim-1	Abcam	Cat#ab47635; RRID: AB_882998
anti-α-SMA	BOSTER	Cat#BM0002; RRID: AB_2811044
anti-CD31	BD Biosciences Pharmingen	Cat#550274; RRID: AB_393571
anti-Areg	Abcam	Cat#ab180722
anti-Survivin(D-8)	Santa Cruz	Cat#sc-17779; RRID: AB_628302
anti-Phospho-LATS1 (Thr1079)	Cell Signaling Technology	Cat#8654; RRID: AB_10971635
anti-COL IV	Abcam	Cat#ab19808; RRID: AB_445160
anti-β-tubulin	Proteintech Group	Cat#10068-1-AP; RRID: AB_2303998
Chemicals, peptides, and recombinant proteins
PGE2	Santa Cruz	Cat#sc-201225
Collagen I, Rat Tail	Corning	Cat#354236
4-hydrazinobenzoic acid	Molbase	Cat#SJ000FFC61940
Polyethylenimine	Polysciences, Inc	Cat#19850
EDC (1-ethyl-3-[3-dimethylaminopropyl]carbodiimide hydrochloride)	Thermo Scientific	Cat#22980
sulfo-NHS(N-hydroxysulfosuccinimide)	Thermo Scientific	Cat#24510
TRIzol reagent	Invitrogen	Cat#15596018
RIPA lysis buffer	Solarbio	Cat#R0010
Hieff® qPCR SYBR® Green Master Mix (No Rox)	Yeasen	Cat#11201ES08
FITC-labeled LTL	Vector Laboratories	Cat# FL-1321-2
DAPI	Beyotime Biotechnology	Cat#C1002
2-(N-morpholino) ethanesulfonic acid	Sangon Biotech	Cat#145224
Biological samples
primary renal tubular epithelial cells	This Paper	N/A
Transgenic mice for C57BL/6 Rosa26mTmG transgenic mice	Jackson Laboratory	Cat#007676
C57BL/6 Sox9-CreERT2 transgenic mice	Jackson Laboratory	Cat#018829
C57BL/6 mice	Laboratory Animal Center of the Academy of Military Medical Science	N/A
Critical commercial assays
PGE2 Enzyme Immunoassay Kit	ArborAssays	Cat#K051-H1
Urea Assay Kit	Nanjing Jiancheng Bioengineering Institute	Cat#C013-1
Creatinine Assay Kit	Nanjing Jiancheng Bioengineering Institute	Cat#C011-1
Cell Counting Kit-8	Beyotime Biotechnology	Cat#C0038
Reverse transcriptase core kit	Takara	Cat#RR037A
Hoechst Staining Kit	Beyotime Biotechnology	Cat#C0003
Oligonucleotides
Bad 5′-AAGTCCGATCCCGGAATCC-3′	This paper	N/A
Bad 5′-GCTCACTCGGCTCAAACTCT-3′	This paper	N/A
Bax 5′-TGAAGACAGGGGCCTTTTTG-3′	This paper	N/A
Bax 5′-AATTCGCCGGAGACACTCG-3′	This paper	N/A
Caspase3 5′-TGGTGATGAAGGGGTC ATTTATG-3′	This paper	N/A
Caspase3 5′-TTCGGCTTTCCAGTCAGA CTC-3′	This paper	N/A
Fas 5′-TATCAAGGAGGCCCATTTTGC-3′	This paper	N/A
Fas 5′-TGTTTCCACTTCTAAACCATGCT-3′	This paper	N/A
Gapdh 5′-AGGTCGGTGTGAACGGATTTG-3′	This paper	N/A
Gapdh 5′-TGTAGACCATGTAGTTGAGG TCA-3′	This paper	N/A
Nonspecific shRNA (sh Ctrl) 5′-CCGG-CA ACAAGATGAAGAGCACCAA-CTCGAG- TTGGTGCTCTTCATCTTGTTG-TTTTTG-3′	Sigma-Aldrich	Cat#SHC002
shRNA-Yap (sh Yap) 5′-CCGG-GCAGAC AGATTCCTTTGTTAA-CTCGAG-TTAAC AAAGGAATCTGTCTGC-TTTTTG-3′	Broad Institute	TRCN0000095864
Software and algorithms
Two-photon microscope system	Olympus	FV1000
Scanning electron microscopy (SEM)	HITACHI	X-650
FT-IR spectromete	Bio-Rad	FTS-6000
Parallel-plate rheometer	New Castle	N/A
Imaris	Oxinst	https://imaris.oxinst.com/
Prism 8	GraphPad	https://www.graphpad.com/scientific-software/prism/
ImageJ	ImageJ	https://imagej.nih.gov/ij