Figure 1.

HHIP-AS1 knockdown inhibited the osteogenic differentiation potential of PDLSCs. (a) Real-time RT-PCR result showed the expression level of HHIP-AS1 at 0, 2, 4, and 6 h under continuous compressive pressure. (b) Real-time RT-PCR result showed the knockdown efficiency of HHIP-AS1 in PDLSCs. (c) ALP activity after 5 days of osteogenic induction. (d) Alizarin Red staining result after 2 weeks of osteogenic induction. (e) Calcium quantitative analysis. (f) Western blot results displayed expressions of BSP, OCN, and OSX after 1 week of osteogenic induction. GAPDH was used as internal control in real-time RT-PCR and Western blot. One-way ANOVA or Student's t-test was performed to determine statistical significance. All error bars represent the SD (n = 3). ^∗P ≤ 0.05; ^∗∗P ≤ 0.01.