Table 3.
Rate of change in CSF protein levels caused by delays in sample processing in cell-free and whole CSF of patients with or suspected of neurological disorders
| Protein | CV |
RCF (%) | SECF | PCF | RWH (%) | SEWH | PWH |
|---|---|---|---|---|---|---|---|
| Intra/inter | |||||||
| CTSH | 5/11 | 56.91 | 3.38 | 0.00107 | 15.16 | 5.25 | 0.0666 |
| ENTPD5 | 9/11 | −6.48 | 0.38 | 0.0053 | −4.3 | 0.45 | 0.0135 |
| WWP2∗ | 6/15 | − 6.93 | 0.45 | 0.0066 | −3.19 | 0.63 | 0.0436 |
| CDHR5∗ | 5/10 | 6.56 | 0.74 | 0.00933 | −0.28 | 1.3 | 0.805 |
| CHRDL2∗ | 6/10 | 1.76 | 0.18 | 0.00974 | −0.03 | 0.47 | 0.937 |
| TYMP | 9/13 | 5.81 | 1.14 | 0.0285 | −1.65 | 1.65 | 0.415 |
| DDC | 5/12 | −2.95 | 0.53 | 0.0343 | −1.67 | 0.48 | 0.078 |
| NPTXR | 6/12 | 1.46 | 0.3 | 0.0369 | 0.28 | 0.5 | 0.652 |
| APEX1∗ | 6/14 | 5.33 | 1.24 | 0.0433 | −4.11 | 1.31 | 0.106 |
| CD2AP | 6/8 | −1.57 | 0.72 | 0.169 | −2.43 | 0.26 | 0.0129 |
| ADA | 5/29 | −1.08 | 1.09 | 0.422 | −1 | 0.14 | 0.0205 |
| RNASE3 | 6/18 | −1.4 | 1.81 | 0.474 | −2.36 | 0.39 | 0.03 |
| NPM1∗ | 4/16 | 7.13 | 7.31 | 0.541 | −14.79 | 0.51 | 0.00561 |
Rates of change (R, %) in protein levels per hour of delay in sample processing as determined by the comparison of samples with baseline (<1 h) and 6 h delay at room temperature (22 °C) are given for both cell-free (CF) and whole (WH) CSF. Only proteins with a significant change (p < 0.05) within 6 h as determined by standard Student's t test are listed. The relative variability in inter/intra runs along with the rate of change for additional proteins, which are provided in supplemental Table S3. Highlighted proteins (∗) showed changes in detectable presence between the two sampled time points; therefore, rate is an approximation.
Abbreviations: ADA, adenosine deaminase; APEX1, DNA-(apurinic or apyrimidinic site) lyase; CD2AP, CD2-associated protein; CDHR5, cadherin-related family member 3; CHRDL2, chordin-like 2; CTSH, cathepsin H; DDC, aromatic-l-amino-acid decarboxylase; ENTPD5, ectonucleoside triphosphate diphosphohydrolase 5; NPM1, nucleophosmin 1; NPTXR, neuronal pentraxin receptor; RNASE3, ribonuclease, RNase A family, 3; TYMP, thymidine phosphorylase; WWP2, WW domain containing E3 ubiquitin protein ligase 2.