Figure 3.

Representative morphology of fibroblasts and directly induced motor neurons (diMNs) at indicated weeks post-viral infection (wpi)

Transduced cells are identified with co-expressed green fluorescent protein (GFP) at early time points (0.5 and 2 wpi). Transduced fibroblasts gradually become neuron-like morphology within 2 wpi. More complicated branches and very long axons can be detected at late developmental stages with immunostaining of tubulin Beta 3 Class III (TUBB3). Scale bars: 100 μm. Adapted from (Ding et al., 2020) with permission.