| Astrocyte medium | Final concentration | Amount |
|---|---|---|
| DMEM | N/A | 42 mL |
| Fetal bovine serum (FBS) | 15% | 7.5 mL |
| Penicillin/Streptomycin (100×) | 1% | 0.5 mL |
| Total | N/A | 50 mL |
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