| Neural induction medium | Final concentration | Amount |
|---|---|---|
| DMEM:F12 (1:1) | N/A | 24 mL |
| Neurobasal media | N/A | 24 mL |
| Penicillin/Streptomycin (100×) | 1% | 0.5 mL |
| N2 (100×) | 1× | 0.5 mL |
| B27 (50×) | 1× | 1 mL |
| FSK (20 mM) | 10 mM | 25 μL |
| DM (10 mM) | 1 mM | 5 μL |
| bFGF (200 μg/mL) | 10 ng/mL | 2.5 μL |
| Total | N/A | 50 mL |