REAGENT and RESOURCES	SOURCE	IDENTIFIER
Experimental models: Cell lines
Human embryonic kidney (HEK) 293T cells	American Type Culture Collection	ATCC Cat# CRL-3216, RRID:CVCL_0063
Healthy skin fibroblasts	Coriell Institute for Medical Research	Coriell Cat# GM04506, RRID:CVCL_7413
Healthy skin fibroblasts	Coriell Institute for Medical Research	Coriell Cat# GM03652, RRID:CVCL_7397
Healthy skin fibroblasts	Coriell Institute for Medical Research	Coriell Cat# GM08400, RRID:CVCL_7483
Mouse Primary Astrocytes	N/A	N/A
Chemicals, peptides, and recombinant proteins
Fetal Bovine Serum (FBS)	Corning	Cat# 35-010-CV
Penicillin/Streptomycin (Pen/Strep)	Thermo Fisher Scientific	Cat# 15140122
Dulbecco's Modified Eagle Medium (DMEM)	Thermo Fisher Scientific	Cat# 11995073
DMEM/nutrient mixture F-12 (DMEM-F12) with HEPES; without L-glutamine	Cytiva	Cat# SH30126.01
Neurobasal medium	Thermo Fisher Scientific	Cat# 21103049
Gelatin	Bio-Rad	Cat# 118860A
Matrigel	Corning	Cat# 354230
B-27 Supplement (50 ×)	Invitrogen	Cat# 17504044
N-2 Supplement (100×)	Invitrogen	Cat# 17502001
Forskolin (FSK)	Sigma Aldrich	Cat# F6886
Dorsomorphin (DM)	EMD Millipore	Cat# 171260
Heat Stable Recombinant Human Basic Fibroblast Growth Factor (bFGF)	Gibco	Cat#PHG0367
Brain Derived Neurotrophic Factor (BDNF)	PeproTech	Cat# 450-02
Glial-Derived Neurotrophic Factor (GDNF)	PeproTech	Cat# 450-10
Neurotrophin-3 (NT3)	PeproTech	Cat# 450-03
Bovine serum albumin (BSA)	Thermo Fisher Scientific	Cat# bp9706
Hoechst 33342 (HST)	Thermo Fisher Scientific	Cat# H3570
Triton X-100 (TX-100)	Thermo Fisher Scientific	Cat# 85111
Paraformaldehyde (PFA)	Sigma Aldrich	Cat# 158127
Trypsin-EDTA (0.25%)	Thermo Fisher Scientific	Cat# 25200056
Polyethyleneimine (PEI), 25 kDa linear	Polysciences	Cat# 23966-2
Polybrene (Hexadimethrine bromide)	Sigma Aldrich	Cat# H9268-10g
Ara-C (Arabinosylcytosine)	Sigma Aldrich	Cat# C1768
Antifade Mounting Medium	Vector Laboratories	Cat#H-1400-10
Na2HPO4	Sigma Aldrich	Cat# S-0876
NaCl	Thermo Fisher Scientific	Cat# S271-10
KCl	Thermo Fisher Scientific	Cat# P-217
K2HPO4.3H2O	Sigma Aldrich	Cat# P-5504
PVA mountain medium	N/A	N/A
DMSO, Dimethyl Sulfoxide	Thermo Fisher Scientific	Cat# D1391
Antibodies
Rabbit anti-tubulin Beta 3 Class III (TUBB3), monoclonal (1:2000)	BioLegend	BioLegend Cat# 801201, RRID:AB_2313773
Chicken anti-microtubule-associated protein 2 (MAP2) (1:10,000), polyclonal	BioLegend	Cat# 822501, RRID:AB_2564858
Goat anti-CHAT, monoclonal (1:200)	Millipore	Millipore Cat# AB144P, RRID:AB_2079751
Rabbit anti-SYN1, monoclonal (1:200)	Cell Signaling Technology	Cat# 5297
Mouse anti-HB9, monoclonal (1:500)	DSHB	Cat# 81.5C10-c
Rabbit anti-Islet1 (ISL1), monoclonal (1:250)	Abcam	Cat# ab109517; RRID: AB_10866454
Donkey anti-Mouse IgG (H + L), Alexa Fluor 488 (1:500)	Jackson ImmunoResearch	Cat# 715-545- 150, RRID: AB_2340846
Sheep anti-Mouse IgG (H + L), Alexa Fluor 594 (1:500)	Jackson ImmunoResearch	Cat# 515-515-062, RRID:AB_2340331
Donkey Anti-Rabbit IgG (H+L), Alexa Fluor 488 (1:500)	Jackson ImmunoResearch	Cat# 711-545-152, RRID:AB_2313584
Donkey Anti-Rabbit IgG (H+L), Alexa Fluor 594 (1:500)	Jackson ImmunoResearch	Cat# 711-585- 152, RRID:AB_2340621
Recombinant DNA
pRSV-Rev	Addgene	RRID:Addgene_12253
pMDLg/pRRE	Addgene	RRID:Addgene_12251
pMD2.G	Addgene	RRID: Addgene_12259
pCSC-NGN2-IRES-GFP-T2A-Sox11	Addgene	RRID:Addgene_90214
pCSC-ISL1-T2A-LHX3	Addgene	RRID:Addgene_90215
Others
15 mL polystyrene Conical tube	Thermo Fisher Scientific	Cat# 14-959-53A
50 mL polystyrene Conical tube	Thermo Fisher Scientific	Cat# 14-432-22
24-well cell culture plates	Thermo Fisher Scientific	Cat#142485
10 cm Cell Culture Dishes	Thermo Fisher Scientific	Cat# 130182
0.45 μm Sterile Filter Unit (PVDF membrane)	EMD Millipore	Cat# SLHVM33RS
0.22-μm vacuum filter	Millipore	Cat# SLGP033RB