| Chemicals, peptides, and recombinant proteins |
|
| Absolute ethanol |
Fisher Scientific |
Cat# E/0650DF/21 |
| Ca2+ and Mg2+-free Hanks’ Balanced Salt solution (HBSS) |
Sigma-Aldrich |
Cat# H6648 |
| Ca2+ and Mg2+-free phosphate-buffered saline (PBS) 10×
|
Euromedex |
Cat# ET 330-A |
| Dimethyl sulfoxide (DMSO) |
Euromedex |
Cat# UD8050-05C |
| Ethylenediaminetetraacetic acid (EDTA) disodium salt 0.5M |
Euromedex |
Cat# EU0084-B |
| Ethylenediaminetetraacetic acid (EDTA) powder |
Euromedex |
Cat# EU0007-B |
| Gelbond® film 124 × 258 mm cut in 3 pieces of 124 × 86 mm |
Cityva |
Cat# 80-1129-32 |
| Low Melting Point (LMP) Agarose |
Sigma-Aldrich |
Cat# A9414 |
| NaCl |
Euromedex |
Cat# 1112-A |
| NaOH pellet |
Euromedex |
Cat# 2020-A |
| SYBR Gold |
Invitrogen |
Cat# S11494 |
| TE buffer 10×
|
Fisher Scientific |
Cat# BP2477-500 |
| Tris base |
Euromedex |
Cat# 26-128-3094-B |
| Triton X-100 |
Euromedex |
Cat# 2000-B |
| Trypan blue |
Gibco |
Cat# 15250061 |
|
| Software and algorithms |
|
| NIS Elements software for image acquisition (F-package version no. 4) |
Nikon |
n/a |
| CometScore 2.0 |
rexhoover.com |
http://rexhoover.com/index.php?id=cometscore |
|
| Other |
|
| 1.5 mL Tubes |
VWR |
Cat# 211-2130 |
| Cover slides 22×60 mm |
Knittel Glass |
Cat# 100267 |
| Cyanoacrylate glue |
Many convenience stores |
n/a |
| Freezing Container, Nalgene® Mr. Frosty |
Sigma-Aldrich |
Cat# C1562-1EA |
| Microscope slides 76×50×1 mm |
Knittel Glass |
Cat# 100008 |
| Scalpel blade N°10 |
VWR |
Cat# 233-5472 |
| Centrifuge |
Eppendorf |
Cat# 5418R |
| Dounce tissue grinder with the loose pestle |
VWR |
Cat# BELC1984-10007 |
| Electrophoresis tank with a platform of 20×20 cm and a distance of 35 cm between the 2 platinum electrodes |
Econo-Submarine SGE 0220-02 |
C.B.S. Scientific, USA |
| Fluorescence microscope with camera |
Nikon microscope equipped with a Luca S camera Source of fluorescence: Nikon C-HGFIE HG Fiber illuminator INTENSILIGHT |
Eclipse 50i |
| Hemocytometer |
VWR |
Cat# HECH40453702 |
| pH meter |
Mettler Toledo |
Cat# SevenCompact pH meter S220 |