FIGURE 1.

Lomerizine 2HCl inhibits proliferation and induces apoptosis of CRC cells. (A) Diagram shows the screening approach. (B) Chemical structure of lomerizine 2HCl is shown. (C) HT‐29 and DLD‐1 cells were treated with lomerizine 2HCl (5, 10, 20, 40, and 80 μM) for 5 days and the cell viability was detected by CCK‐8 with DMSO as control. (D) Colony formation assay shows the effects of lomerizine 2HCl on colony formation. (E) Flow cytometry analysis shows the effects of lomerizine 2HCl (DMSO, 10, 20, and 40 μM) on cell apoptosis at 48 h. (F) Cells were treated with lomerizine 2HCl for 48 h and the cell lysates were collected to analyze apoptosis‐related proteins. All data shown represent three independent experiments. Bars, SD; *p < 0.05, **p < 0.01, ***p < 0.001