FIGURE 4.

Demethylzeylasteral decreases the stability of c‐Myc protein by FBXW7. (A) The effect of Dem and MG132 on c‐Myc expression in GC cells was tested by Western blot. (B) The ubiquitination of c‐Myc in 293‐FT cells was enhanced after Dem treatment. (C) The mRNA level of FBXW7 and SKP2 in GC cells after Dem treatment was detected by qPCR. (D) The expression of FBXW7 and c‐Myc in GC cells after Dem treatment was detected by Western blot. (E) The effects of FBXW7 knockdown on protein expression in Dem treated cells were analyzed by Western blot. ***p < 0.001, **p < 0.01, *p < 0.05