FIGURE 5.

Demethylzeylasteral suppresses clonogenicity and tumor growth of GC through down‐regulating the expression of c‐Myc. (A) Soft agar assay was performed with c‐Myc or empty vector overexpressed GC cells after DMSO or Dem treatment, scale bar = 100 µm. (B) The numbers of clones in panel (A) was counted. (C) Tumor volume and photograph of tumors from indicated mice treated with DMSO or Dem were shown. (D) Tumor weights of indicated mice treated with DMSO or Dem were shown. (E) The weights of mice in each group were measured. (F) c‐Myc overexpression in indicated tumors was investigated by IHC, scale bar = 100 µm. (G) Summary of the mechanism by which Dem inhibits cell proliferation and tumor progression in human GC. ***p < 0.001, **p < 0.01, *p < 0.05