FIGURE 6.

Demethylzeylasteral enhances GC cell chemosensitivity to Doxorubicin and 5‐Fluorouracil in vitro. (A and B) Growth curve of GC cells with different treatment (1 µM Dem, 0.1 µM DOX, 150 µM 5‐Fu, 1 µM Dem+0.1 µM DOX, 1 µM Dem+150 µM 5‐Fu) was shown. The efficiency index (q) analysis of Dem combined with 5‐Fu or DOX treatment was determined through Jin's formula. (C) BrdU staining assay was performed in different drug combination groups, scale bar = 100 µm. (D) BrdU‐positive rates in panel (C) were quantified. (E) Clone formation assay was performed in different drug combination groups. (F) Clone numbers in panel (E) were quantified. (G) Apoptosis in different drug combination groups was detected. (H) Apoptotic rates in panel (G) were quantified. (I) The apoptotic proteins expression of were detected by Western blot. ***p < 0.001, **p < 0.01, *p < 0.05