Fig. 7.

Rapamycin preserves mitochondrial membrane potential in cardiomyocytes of diabetic mice (db/db) following SI/RO. Isolated murine cardiomyocytes from C57 and db/db mice were subjected to SI/RO. During RO (1 h), cardiomyocytes were treated with rapamycin (RAPA, 100 nM) or DMSO (solvent of RAPA). Cardiomyocyte membrane potential was examined by using cationic lipophilic probe JC-1. Quantification of JC-1 aggregate (red/orange color)/monomer (in green color) ratio indicates the preservation of mitochondrial membrane potential