At PND 21, sera and livers of dams exposed to PFAS during gestation and lactation were collected. A) Serum alanine transaminase (ALT) was measured via colorimetric assay, B) Total lipids were isolated and normalized to tissue weight. C) Triglycerides, and B) cholesterol content, and was measured via colorimetric assay and normalized to tissue weight. Calculations were performed using a two-way ANOVA followed by Fisher’s LSD test. All values are means ± SEM; N = 3–5 litters. * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001, and **** p ≤ 0.0001.