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. Author manuscript; available in PMC: 2022 Oct 1.
Published in final edited form as: Toxicology. 2021 Aug 28;462:152921. doi: 10.1016/j.tox.2021.152921

Figure 4. Perinatal PFAS and high fat diet effects pup liver lipid content At PND 21.

Figure 4.

Frozen livers of pups exposed to PFAS during gestation and lactation were collected at PND 21. A) Total lipids were isolated and normalized to tissue weight. B) Triglycerides, and C) cholesterol content, and was measured via colorimetric assay and normalized to tissue weight. No significant differences in lipid content were observed between male and female pups, and data presented represent the average of both male and female pups per litter. Calculations were performed using a two-way ANOVA followed by Fisher’s LSD test. All values are means ± SEM; N = 3–5 litters. * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001, and **** p ≤ 0.0001.