. Author manuscript; available in PMC: 2022 Oct 1.

Published in final edited form as: Toxicology. 2021 Aug 28;462:152921. doi: 10.1016/j.tox.2021.152921

Table 1:

Maternal and Offspring Body and Liver Weights.

	Parameter	SD+Veh	HFD+Veh	SD+PFOA	HFD+PFOA	SD+PFOS	HFD+PFOS	SD+PFHxS	HFD+PFHxS	SD+PFAS mix	HFD+PFA mix

Maternal Endpoints	# of Live Births per litter	12.2 ± 1.8	13.6 ± 1.2	14.2 ± 0.6	10.0 ± 1.3 ^#¶	11.4 ± 1.0	13.0 ± 0.9	12.6 ± 1.1	14.4 ± 0.7	14.3 ± 1.2	12.3 ± 1.4
	BW at PND 21 (g)	31.8 ± 1.00	35.2 ± 0.68 ^*	35.2 ± 1.10 ^*	32.1 ± 1.10 ^#¶	31.9 ± 0.41	34.3 ± 0.82	31.9 ± 0.44	34.8 ± 0.78 ^*^¶	33.4 ± 1.43	34.0 ± 0.70
	Liver Weight (g)	1.67 ± 0.09	1.79 ± 0.06	3.28 ± 0.20 ^*	2.44 ± 0.19 ^*^#¶	1.74 ± 0.19	1.53 ± 0.05	1.94 ± 0.10	1.89 ± 0.13	3.20 ± 0.39 ^*^ǂ†	3.04 ± 0.13 ^*^#§ǂ†
	Liver: BW ratio (%)	5.07 ± 0.12	5.08 ± 0.18	9.27 ± 0.67 ^*	7.32±0.34 ^*^#¶	5.46 ± 0.14	4.49 ± 0.32	6.09 ± 0.21	5.42 ± 0.26	9.58 ± 1.07 ^*^ǂ†	8.36 ± 0.09 ^*^#§ǂ†

Neonatal (PND 5)	BW (g) ^**	3.3 ± 0.2	3.6 ± 0.2	3.2 ± 0.1	3.7 ± 0.2	3.5 ± 0.5	3.6 ± 0.1	3.5 ± 0.2	3.7 ± 0.2	3.1 ± 0.2	4.0 ± 0.2 ^*^¶

Pup Endpoints at PND 21	Male BW (g)	14.6 ± 0.15	16.8 ± 0.40 ^*	13.1 ± 0.73	15.3 ± 0.72 ^¶	13.7 ± 0.81	16.6 ± 0.30 ^*^¶	14.0 ± 0.47	17.4 ± 0.39 ^*^¶	13.6 ± 0.42	16.1 ± 0.90 ^¶
	Female BW (g)	13.7 ± 0.31	16.0 ± 0.33 ^*	12.6 ± 0.55	14.3 ± 0.73 ^#¶	13.1±0.67	15.8±0.34 ^*^¶	13.8 ± 0.42	16.6 ± 0.33 ^*	13.3 ± 0.52	15.1 ± 0.91 ^¶
	Liver Weight (g) ^**	0.649 ± 0.017	0.712 ± 0.029	0.711 ± 0.034	0.908 ± 0.043 ^*^#¶	0.564 ± 0.051	0.664 ± 0.027	0.593 ± 0.029	0.676 ± 0.015	0.672 ± 0.036	0.897 ± 0.044 ^*^¶#ǂ†
	Liver: BW ratio (%) ^**	4.49 ± 0.11	4.40 ± 0.12	5.50 ± 0.13 ^*	5.79 ± 0.21 ^*^#	4.16 ± 0.17	4.03 ± 0.10 ^*	4.20 ± 0.09	3.99 ± 0.08 ^*^#	4.92 ± 0.10 ^§ǂ†	5.84±0.28 ^*^¶#ǂ†

Pup Endpoints at PND 90	Male BW (g)	42.5 ± 1.06	57.3 ± 2.46 ^*	41.6 ± 2.09	60.0 ± 2.56 ^*^¶	43.4 ± 0.37	59.5 ± 4.33 ^*^¶	40.1 ± 2.97	56.9 ± 1.00 ^*^¶	40.5 ± 1.07	58.7 ± 1.36^*^¶
	Male Liver Weight (g)	1.78 ± 0.04	2.59 ± 0.29 ^*	1.79 ± 0.12	2.80 ± 0.29 ^*^¶	1.72 ± 0.03	2.64 ± 0.47 ^*^¶	1.44 ± 0.08	2.24 ± 0.12 ^¶	1.64 ± 0.11	2.38 ± 0.22 ^¶
	Male Liver: BW ratio (%)	4.19 ± 0.11	4.47 ± 0.31	4.29 ± 0.23	4.62 ± 0.36	3.96 ± 0.11	4.37 ± 0.51	3.63 ± 0.13	3.91 ± 0.19	4.04 ± 0.17	4.03 ± 0.34
	Female BW (g)	28.5 ± 1.49	45.5 ± 3.19 ^*	33.2 ± 1.47	42.8 ± 2.87 ^*^¶	31.2 ± 3.50	43.8 ± 5.14 ^*^¶	33.5 ± 0.83	42.8 ± 1.86 ^*^¶	32.7 ± 1.59	39.4 ± 7.01^*
	Female Liver Weight (g)	1.18 ± 0.11	1.52 ± 0.15	1.34 ± 0.11	1.43 ± 0.14	1.20 ± 0.11	1.73 ± 0.15 ^*^¶	1.34 ± 0.03	1.36 ± 0.05	1.35 ± 0.13 ^ǂ	1.19 ± 0.07 ^ǂ
	Female Liver: BW ratio (%)	4.13 ± 0.21	3.32 ± 0.13 ^*	4.19 ± 0.20	3.35 ± 0.15 ^*^¶	3.90 ± 0.11	4.15 ± 0.86 ^#	4.00 ± 0.12	3.20 ± 0.07 ^*^¶	4.12 ± 0.22	3.15 ± 0.33 ^*^¶ǂ

Timed-pregnant female CD-1 mice were dosed with either vehicle (0.5% Tween 20 in water), 1 mg/kg PFOA, 1 mg/kg PFOS, 1 mg/kg PFHxS, or a PFAS mixture (1 mg/kg of each PFOS, PFOA, and PFHxS) via oral gavage (10 mL/kg) from gestation day (GD) 1 through postnatal day (PND) 21. Maternal body weights (BW) and litter sizes were recorded. At PND 5, neonatal body weights were measured, and litters were culled to 10 pups. At PND 21, dams and some pups from each dam were euthanized, and liver weights were determined. Remaining pups continued the same diet as dam and were euthanized at 13 week of age, and liver weights were determined. Calculations were performed using a two-way ANOVA followed by Fisher’s LSD test.

^**

no differences were observed between male and female pups for this timepoint, and data presented represent the average of both male and female pups per litter. All values are means ± SEM; N = 3–5 dams or litters.

p<0.05 versus SD+Veh

p<0.05 versus HFD+Veh.

^¶

p<0.05 SD versus HFD within each treatment (i.e., SD+PFOA vs HFD+PFOA)

^§

p<0.05 versus PFOA and PFAS mix within each diet (i.e., SD+PFOA vs SD+PFAS mix)

^ǂ

p<0.05 versus PFOS and PFAS mix within each diet (i.e., SD+PFOS vs SD+PFAS mix)

^†

p<0.05 versus PFHxS and PFAS mix within each diet (i.e., SD+PFHxS vs SD+PFAS mix).