Table 4:
PND 21 Pup Liver PFAS Concentrations and liver to serum ratios.
| SD+Veh | HFD+Veh | SD+PFOA | HFD+PFOA | SD+PFOS | HFD+ PFOS | SD+PFHxS | HFD+PFHxS | SD+PFAS mix | HFD+PFAS mix | ||
|---|---|---|---|---|---|---|---|---|---|---|---|
|
| |||||||||||
| Pups at 3 weeks of age(PND 21) ** | PFOA (μg/g tissue) | <LLOQ | <LLOQ | 26.41 ± 2.07 | 35.73 ± 2.57 * | - | - | - | - | 18.79 ± 3.22 | 33.04 ± 4.00 |
| PFOS (μg/g tissue) | <LLOQ | <LLOQ | - | - | 1.41 ± 0.09 | 1.30 ± 0.18 | - | - | 0.91 ± 0.07 # | 1.11 ± 0.06 | |
| PFHxS (μg/g tissue) | <LLOQ | <LLOQ | - | - | - | - | 4.54 ± 0.20 | 4.79 ± 0.44 | 6.03 ± 0.81 | 5.69 ± 0.59 | |
|
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| Liver to Serum ratios | PFOA (μg/mL:μg/g tissue) | - | - | 2.3 ± 0.22 | 2.1 ± 0.20 | - | - | - | - | 1.6 ± 0.18 | 2.2 ± 0.28 |
| PFOS (μg/mL:μg/g tissue) | - | - | - | - | 2.3 ± 0.57 | 3.0 ± 0.60 | - | - | 3.2 ± 0.27 | 3.4 ± 0.79 | |
| PFHxS (μg/mL:μg/g tissue) | - | - | - | - | - | - | 0.17 ± 0.011 | 0.20 ± 0.012 | 0.25 ± 0.019 # | 0.22 ± 0.017 | |
Timed-pregnant female CD-1 mice were dosed with either vehicle (0.5% Tween 20 in water), 1 mg/kg PFOA, 1 mg/kg PFOS, 1 mg/kg PFHxS, or a PFAS mix (1 mg/kg of each PFOS, PFOA, and PFHxS) via oral gavage (10 mL/kg) from gestation day (GD) 1 through postnatal day (PND) 21. PFAS were extracted from pup serum collected at PND 21, and PFAS were quantified using LC-MS/MS. All mice that were not dosed with PFAS had concentrations below the lower limit of quantification (LLOQ). LLOQ were 0.14 μg/g for PFOA, 0.14 μg/g for PFOS, and 0.14 μg/g for PFHxS.
no differences were observed between male and female pups for this timepoint, and data presented represent the average of both male and female pups per litter. Calculations were performed using a one-way ANOVA followed by Fisher’s LSD test. All values are means ± SEM; N = 3–5 litters.
p<0.05 between diet treatment within the same compound (i.e., SD+PFOS vs PFOS HFD)
p<0.05 between single compound treatment and the PFAS mixture with same diet (i.e., SD+PFOS vs SD+PFAS mix).