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Examples of confocal laser scanning micrographs of bacteria in material removed by ultrasonication (sonicate) from culture-negative hip prostheses to illustrate coccoid cells present singly and in small groups (a) and in a large aggregate (b). Bacteria were labelled with anti-Staphylococcus spp. polyclonal antiserum and an anti-P. acnes-specific MAb, followed by labelling with suitable fluorescently conjugated secondary antibodies.
