TABLE 2.

Bacteria detected from culture-positive retrieved prosthetic hip implants by culture, IFM, and bacterial 16S rRNA gene detection and associated tissue inflammatory response

Sample no.	Bacterium(a) isolated	Tissue infiltration by inflammatory cells (score)a			IFM result		16S rRNA gene detection
		PMNb	LYMc	MACd	Morphology	Scoree
1	S. epidermidis	1	1	2	Cocci	3	+
2	S. epidermidis	2	1	3	Cocci	3	+
3	S. aureus	0	1	0	Cocci	2	+
4	S. capitis	0	0	1	Cocci	1	+
5	S. epidermidis	0	1	2	Cocci and P. acnes	2	+
6	S. hominis	0	1	2	Cocci and P. acnes	1	+
7	S. epidermidis	1	1	3	P. acnes	2	+
8	S. epidermidis and S. hominis	0	2	3	Cocci	2	+
9	S. epidermidis and S. hominis	1	2	3	Cocci	2	+
10	S. epidermidis and S. capitis	2	2	2	Cocci and P. acnes	1	+
11	P. acnes	2	2	3	P. acnes	1	+
12	P. acnes	1	1	1	P. acnes	1	+
13	P. acnes	NSf	NS	NS	P. acnes	3	+
14	P. acnes	0	2	3	Cocci and P. acnes	2	+
15	P. acnes	0	1	2	Cocci and P. acnes	2	+
16	P. acnes	2	2	3	Cocci and P. acnes	1	+
17	P. acnes	NS	NS	NS	Cocci and P. acnes	3	+
18	P. acnes	NS	NS	NS	Cocci and P. acnes	3	+
19	P. acnes	NS	NS	NS	Cocci and P. acnes	1	+
20	P. acnes	NS	NS	NS	Cocci and P. acnes	1	+
21	P. acnes	NS	NS	NS	Cocci and P. acnes	1	+
22	P. acnes	0	1	2	NDg	ND	+
23	S. haemolyticus and P. acnes	1	1	1	Cocci and P. acnes	1	+
24	Micrococcus sp. and P. acnes	1	2	3	Cocci and P. acnes	1	+
25	S. epidermidis and P. acnes	NS	NS	NS	Cocci	1	+

^aNumber of inflammatory cells per high-power field: 0, no cells; 1, 1 to 10 cells; 2, 10 to 20 cells; 3, 20 or more cells. 

^bPMN, polymorphonuclear leukocyte. 

^cLYM, lymphocyte. 

^dMAC, tissue macrophage. 

^eNumber of bacteria per well: 0, no bacteria; 1, 1 to 10 bacteria; 2, 10 to 50 bacteria; 3, 50 or more bacteria. 

^fNS, not suitable for histological examination. 

^gND, not done due to insufficient sample volume.