Table 2. List of reagents used to prepare 500 ml of stock media.

	Reagents	Volume
1	DMEM w/ F12	450 ml
2	FBS	50 ml
3	L-glutamine (100×)	5 ml
4	Pen/Strep (100×)	5 ml
5	MEM-NEAA (100×)	5 ml
6	Chemically defined Lipid Concentrate (100×)	5 ml
7	Gentamicin (50 mg/ml)	1 ml
	MEM Vitamin Solution (100×)	5 ml

Note: To make complete media, add the following supplements in Table 3 on the day staring cell culture.