Table 2.
Results of genotyping using the MST method
| No. | Herd ID | Voivodeship | Type of sample | Ct real-time PCR |
Alleles identified in spacersb | Sequence type | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Cox 2 | Cox 5 | Cox 18 | Cox 20 | Cox 22 | Cox 37 | Cox 51 | Cox 56 | Cox 57 | Cox 61 | ||||||
| 1. | B1 | Kuyavia-Pomerania | BTM | 29.27 | 3 | – | 6 | 1 | – | 10 | 4 | 10 | – | 5 | 61a |
| 2. | C1 | Lublin | tissue | 29.28 | 3 | 2 | 6 | 1 | 5 | 10 | 4 | 10 | 6 | 5 | 61 |
| 3. | I1 | Greater Poland | BTM | 30.94 | 3 | 2 | – | – | 5 | 10 | 4 | 10 | 6 | 5 | 61/74a |
| 4. | I2 | Greater Poland | BTM | 31.18 | 3 | 2 | 6 | – | 5 | 10 | – | 10 | – | 5 | 61a |
| 5. | I3 | Greater Poland | BTM | 31.19 | 3 | 2 | 6 | – | 5 | 10 | 4 | 10 | 6 | 5 | 61a |
| 6. | I4 | Greater Poland | BTM | 29.76 | 3 | 2 | 6 | 1 | 5 | 10 | 4 | 10 | – | 5 | 61a |
| 7. | I5 | Greater Poland | BTM | 29.36 | 3 | 2 | 6 | 1 | 5 | 10 | 4 | 10 | – | 5 | 61a |
| 8. | I6 | Greater Poland | BTM | 30.98 | 3 | – | – | 1 | 5 | 10 | – | 10 | – | 5 | 61/74a |
| 9. | J1 | West Pomerania | BTM | 29.6 | 3 | 2 | 6 | 1 | – | 10 | – | 10 | – | 5 | 61a |
a Sequence type established based on incomplete allelic profile
bAccording to an online database [25]