Table 2.

Fluorescence in situ hybridization (FISH) on sorted blasts

Biobank number	Cytogenetic details	FISH pre-chemotherapy	FISH post-chemotherapy	Probe used
64	46, XY, inv.(3)(q21q26), del(7)(q22) [10]	Failed	Insufficient material	NA
121	45, XX, inv.(3)(q21q26), −7[8]/46, XX [2]	44/45 1G1O 1/45 1G	83/120 1G1O 37/120 1G	Vysis D7S522/CEP7
205	44, XX, add(3)(p25), −5, −7[12]	61/100 1G1O 39/100 1G	Insufficient material	Vysis D7S522/CEP7
285	Normal
349	Normal
494	45 ~ 49, XY, −4, −5, −7, del(9)(q?22),? der(15;17) (q10;q10), + 21, del(22)(q13), + 2 ~ 5mar[cp10]	Insufficient material	92/100 1G1O 8/100 1G	Vysis D7S522/CEP7

Where sufficient cells were available, FISH was used to confirm clonality of sorted blast populations. Samples 285 and 349 had normal cytogenetics and no target for FISH. Samples 121, 205 and 494 had monosomy 7 which was detected using two probes: CEP7 (green probe targeting the centromere, 7p11.1-q11.1) and D7S522 (orange probe targeting 7q31). 1G1O, 1 green and 1 orange signal per cell; 1G, 1 green signal per cell