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. 2021 Oct 28;14:142. doi: 10.1186/s13048-021-00904-y

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — The abundance of different transcripts from genes affected by the elevated level of NEFAs (upregulated: AGPS, MAP3K8, and TLR6 and downregulated: AGO1, IL33, and GAPDH) was analyzed by real-time RT-qPCR. The RT-qPCR results showed that MAP3K8, TLR6, AGO1, IL33, and GAPDH had the same direction as in the microarray data, and two of them (TLR6 and GAPDH) showed significant differences relative to the respective controls. * represents p-value < 0.05, ** represents p-value < 0.01. GC samples were analyzed after IVM under the high level of NEFAs throughout the IVM culture or control treatment. NC is the control group, and FA is the group exposed to the elevated level of NEFAs. AGPS: Alkylglycerone Phosphate Synthase; MAP3K8: Mitogen-Activated Protein Kinase Kinase Kinase 8; TLR6: Toll Like Receptor 6; AGO1: Argonaute RISC Component 1; IL33: Interleukin 33; GAPDH: Glyceraldehyde-3-Phosphate Dehydrogenase