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. 2021 Oct 28;14:142. doi: 10.1186/s13048-021-00904-y

Table 1.

Information on RT-qPCR primers

Target gene Accession number Forward primer (5’-3’) Reverse primer (5’-3’) Product size Annealing Temperature
AGPS XM_021075128.1 GACGCAGACTTATGATGCAGG AGCCACTGCTTCCGTAACT 180 bp 57 °C
MAP3K8 XM_021064737.1 ATGGAACCGTGGAGGACTTG CGGAGCCGATATTCCTGTAAGT 192 bp 57 °C
TLR6 NM_213760.2 CTCTCATGGCACAGCGAACT CACATCATCCTCTTCAGCGACT 125 bp 59 °C
AGO1 XM_005665209.3 ACGCTGTTACCTCACTGGATAG GGCCAGATGTGACAAGATGAGG 244 bp 59 °C
IL33 NM_001285978.1 ATGGTGGTGGTGATCATCGGA ACACTCCAGGATGGCTCTTACA 201 bp 59 °C
GAPDH NM_001206359.1 ACTGGTGTCTTCACGACCAT GGAGGCATTGCTGACGATCT 159 bp 57 °C
STAR NM_213755.2 CCCTTGGACAGGAGCTGAAC GGCCAGATCTTGGTCACTGT 134 bp 57 °C
CYP11A1 NM_214227.1 AGACACTGAGACTCCACCCCA GACGGCCACTTGTACCAATGT 110 bp 57 °C
HSD3B1 NM_001004049.2 GATCGTCCACTTGTTGCTGG GATCTTGCTCTGGAGCTTAGAA 109 bp 57 °C
CYP19A3 NM_214431.1 GTGGACGTGTTGACCCTCAT TATGCACGAGGGCACTTTCG 94 bp 57 °C
SDHA [61] XM_021076930.1 CACACGCTTTCCTATGTCGATG TGGCACAGTCAGCTTCATTC 94 bp 57 °C
PPIA [61] NM_214353.1 AAAACTTCCGTGCTCTGAGC TTATGGCGTGTGAAGTCACC 112 bp 59 °C

Note: The superscript number beside a target gene indicates the reference from which the primer sequence was obtained