Table 1.
Target gene | Accession number | Forward primer (5’-3’) | Reverse primer (5’-3’) | Product size | Annealing Temperature |
---|---|---|---|---|---|
AGPS | XM_021075128.1 | GACGCAGACTTATGATGCAGG | AGCCACTGCTTCCGTAACT | 180 bp | 57 °C |
MAP3K8 | XM_021064737.1 | ATGGAACCGTGGAGGACTTG | CGGAGCCGATATTCCTGTAAGT | 192 bp | 57 °C |
TLR6 | NM_213760.2 | CTCTCATGGCACAGCGAACT | CACATCATCCTCTTCAGCGACT | 125 bp | 59 °C |
AGO1 | XM_005665209.3 | ACGCTGTTACCTCACTGGATAG | GGCCAGATGTGACAAGATGAGG | 244 bp | 59 °C |
IL33 | NM_001285978.1 | ATGGTGGTGGTGATCATCGGA | ACACTCCAGGATGGCTCTTACA | 201 bp | 59 °C |
GAPDH | NM_001206359.1 | ACTGGTGTCTTCACGACCAT | GGAGGCATTGCTGACGATCT | 159 bp | 57 °C |
STAR | NM_213755.2 | CCCTTGGACAGGAGCTGAAC | GGCCAGATCTTGGTCACTGT | 134 bp | 57 °C |
CYP11A1 | NM_214227.1 | AGACACTGAGACTCCACCCCA | GACGGCCACTTGTACCAATGT | 110 bp | 57 °C |
HSD3B1 | NM_001004049.2 | GATCGTCCACTTGTTGCTGG | GATCTTGCTCTGGAGCTTAGAA | 109 bp | 57 °C |
CYP19A3 | NM_214431.1 | GTGGACGTGTTGACCCTCAT | TATGCACGAGGGCACTTTCG | 94 bp | 57 °C |
SDHA [61] | XM_021076930.1 | CACACGCTTTCCTATGTCGATG | TGGCACAGTCAGCTTCATTC | 94 bp | 57 °C |
PPIA [61] | NM_214353.1 | AAAACTTCCGTGCTCTGAGC | TTATGGCGTGTGAAGTCACC | 112 bp | 59 °C |
Note: The superscript number beside a target gene indicates the reference from which the primer sequence was obtained