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. 2021 Oct 27;25:35. doi: 10.1186/s40824-021-00233-7

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3 — Assessment of osteoinductivity of BMP-2 released from PLA/Biogel scaffold. a Releasing profile of BMP-2 from cage and cage/Biogel. About 90% of BMP-2 was released in the first 3 h from the PLA cage, while the 85% of the BMP-2 was released in 4 days in the PLA cage/Biogel group. (n = 2) b Cell viability assessment. The PLA or Biogel showed no significant cytotoxic effects on mMSCs. (n = 2) c ALP assay result. The results showed the ALP activity of PLA cage/Biogel/BMP-2 group was comparable with BMP-2 group. (n = 2) d, ALP staining result. The PLA cage/Biogel/BMP-2 group showed comparable ALP staining with BMP-2 and PLA cage/Biogel group. Scale bar: 250 μm. e, RT-PCR results. The PLA cage/Biogel/BMP-2 group showed comparable transcription levels of ALP, Runx-2, BSP and OCN. (n = 2)