Table 1.
The HPLC method validation for simultaneous quantification of the four markers in UTE and URE
| Markers | Regression equation | Linear ranges (μg/mL) | r2 | LOD (μg/mL) | LOQ (μg/mL) | Precision (RSD%) | Repeatability (RSD%) | Stability (RSD%) | Recovery (UTE) | Recovery (URE) | ||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| UTE | URE | UTE | URE | Mean (%) | RSD (%) | Mean (%) | RSD (%) | |||||||
| Rhynchophylline | y = 211.5x − 252.6 | 10–1000 | 0.9998 | 1.91 | 9.15 | 2.77 | 2.79 | 1.75 | 2.05 | 1.14 | 100.98 | 1.50 | 97.98 | 1.73 |
| Isorhynchophylline | y = 223.7x − 2403 | 10–1000 | 0.9995 | 2.09 | 10.26 | 1.62 | 1.95 | 2.31 | 1.32 | 2.15 | 99.27 | 1.35 | 99.30 | 0.57 |
| Corynoxeine | y = 152.6x + 1399 | 10–1000 | 0.9997 | 1.83 | 9.07 | 1.83 | 2.46 | 2.71 | 2.09 | 1.62 | 100.63 | 1.45 | 102.84 | 0.73 |
| Isocorynoxeine | y = 107.7x − 140.4 | 10–500 | 0.9999 | 2.03 | 10.22 | 2.54 | 1.80 | 2.57 | 1.05 | 2.04 | 101.62 | 1.46 | 103.19 | 1.30 |
x, concentration in μg/mL; y, peak area; r2, correlation coefficient of regression equations