FIG. 6.

Parallel changes in PFGE profile and lytA hybridization pattern in selected strains of S. pneumoniae. Lanes 1 and 15 were loaded with low-range PFGE markers. Lanes 2, 9, and 14 contain SmaI fragments of the laboratory strain R6 used as molecular size markers. Strains GA 34, GA 39, GA 27, GA 26, FL 10, and FL 21 are from a United States strain collection (4). Strains HIM 15 and HIM 82 are from Mexico (5). Strains SW 96 and SW 95 are from Sweden. (A) SmaI digest of total DNA separated by PFGE. (B) Hybridization of a Southern blot of the gel in panel A with the lytA gene probe. Numbers in the center indicate molecular sizes in kilobases.