TABLE 1.
Variation in lytA hybridization patterns of penicillin-resistant S. pneumoniae isolatesa
| Strain | Serotype | PFGE type | PEN MIC (μg/ml) | Susceptibility or resistance
|
SmaI hybridization pattern (kb)
|
|||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| ERY | TET | CMP | SXT | ermB | mef | tetM | cat | lytAb | ||||
| OH 4 | 6 | 9 | 2 | R | S | S | R | — | 225 | — | — | 100/60 |
| OH 11 | 6 | 9 | 2 | R | S | S | R | — | 225 | — | — | 100/60 |
| OH 12 | 6 | 9 | 2 | R | S | S | R | — | 225 | — | — | 100/60 |
| OH 13 | 6 | 9 | 2 | R | S | S | R | — | 225 | — | — | 100/60 |
| CA 14 | 19 | 3 | 2 | R | R | S | I | — | 115 | 115 | — | 115 |
| TX 4 | 19 | 3 | 2 | R | R | S | R | — | 115 | 115 | — | 115 |
| CA 37 | 19 | 3 (2) | 3 | R | R | S | R | — | 115 | 115 | — | 115 |
| CA 40 | 19 | 3 (2) | 2 | R | R | S | R | — | 115 | 115 | — | 115 |
| TX 8 | 19 | 3 (3) | 2 | R | R | S | I | — | 115 | 115 | — | 115 |
| CA 45 | 23 | 5 (3) | 1.5 | S | S | S | I | — | — | — | — | 260/85/70 |
| CA 8 | 23 | 5 (2) | 2 | S | S | S | R | — | — | — | — | 260/85/70 |
| KS 11 | 23 | 5 | 4 | S | S | S | R | — | — | — | — | 260/85 |
| KY 15 | 23 | 5 | 6 | S | S | S | I | — | — | — | — | 260/85 |
| KY 4 | 23 | 5 | 2 | S | S | S | I | — | — | — | — | 260/85 |
| TX 5 | 14 | 16 | 6 | R | R | S | R | 390 | — | 390 | — | 95/40 |
| GA 30 | NT | 16 | 8 | R | R | S | R | 390 | — | 390 | — | 95/40 |
| KS 10 | 14 | 16 (2) | 6 | R | R | S | R | 390 | — | 390 | — | 95/70 |
| TN 13 | 14 | 16 (2) | 3 | R | R | S | R | 390 | — | 390 | — | 95/70 |
| SW 224 | 9 | B (6) | 1 | S | S | S | R | ND | ND | ND | ND | 260/130 |
| SW 225 | 9 | B (6) | 1 | S | S | S | R | ND | ND | ND | ND | 260/130 |
| SW 226 | 9 | B | 1 | S | S | S | R | ND | ND | ND | ND | 260/95 |
| SW 228 | 9 | B | 1 | S | S | S | R | ND | ND | ND | ND | 260/95 |
| HIM 76 | 23 | A | 2 | R | R | R | R | ND | ND | 390 | ND | 90/30 |
| COL 252 | 23 | A (3) | 2 | S | R | R | I | ND | ND | 390 | ND | (175)/90/30 |
| GA 38 | 23 | A (2) | 2 | R | R | R | R | 390 | — | 390 | 390 | (180)/90/30 |
| TX 16 | 23 | A (2) | 2 | R | R | R | R | 390 | — | 390 | 390 | (180)/90/30 |
| AL 4 | 23 | A | 1.5 | R | R | R | R | 390 | — | 390 | 390 | 90/30 |
| TN 3 | 23 | A (2) | 1.5 | R | R | R | I | 390 | — | 390 | 390 | (180)/90/30 |
| Ark 3 | 23 | A (2) | 3 | R | R | R | R | 390 | — | 390 | 390 | (180)/90/30 |
| FL 4 | 23 | A (2) | 4 | R | R | R | I | — | 390 | 390 | 390 | (180)/90/30 |
| CA 2 | 23 | A (3) | 2 | R | R | R | R | — | 390 | 390 | 390 | (175)/90/30 |
| NY 11 | 23 | A | 2 | R | R | R | R | — | 390 | 390 | 390 | 90/30 |
| KS 4 | 23 | A (3) | 3 | R | R | R | R | — | 390 | 390 | 390 | (175)/90/30 |
| CMN 38 | 9A | B (4) | 2 | S | S | S | R | ND | ND | ND | ND | 260/95 |
| Col 189 | 9V | B | 2 | S | S | S | R | ND | ND | ND | ND | 260/95 |
| UR 219 | 14 | B | 2 | S | S | S | R | ND | ND | ND | ND | 260/95 |
| CA 24 | 9 | B (3) | 2 | S | S | S | R | — | — | — | — | 260/95 |
| CA 52 | 9 | B (2) | 1.5 | S | S | S | R | — | — | — | — | 260/95 |
| TX 9 | 9 | B | 1.5 | S | S | S | R | — | — | — | — | 260/95 |
| TX 14 | 9 | B | 3 | S | S | S | R | — | — | — | — | 260/95 |
| FL 17 | 9 | B (2) | 3 | S | S | S | R | — | — | — | — | 260/95 |
| GA 13 | 9 | B | 1.5 | S | S | S | R | — | — | — | — | 260/95 |
| OH 20 | 9 | B (5) | 1.5 | S | S | S | R | — | — | — | — | 260/95 |
| TN 16 | 9 | B | 1.5 | S | S | S | R | — | — | — | — | 260/95 |
a
Strain numbers and PFGE assignments are as in references 4 and 18. —, no hybridization signal. Abbreviations: PEN, penicillin; ERY, erythromycin; TET, tetracycline; CMP, chloramphenicol; SXT, trimethoprim-sulfamethoxazole; NT, nontypeable; ND, not done; S, susceptible; R, resistant; I, intermediate.
b
Numbers in parentheses refer to unstable bands.