Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Oct 29;14:178. doi: 10.1186/s13045-021-01194-z

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

PMC Copyright notice

Fig. 6 — PLA2G16 is crucial for lncROPM-driven BCSCs formation. A, B qRT-PCR analysis of PLA2G16 expressions in non-BCSCs and BCSCs from various breast cancer cells (A) and clinical breast cancer samples (n = 30) (B). C Western blotting to determine PLA2G16 protein levels in non-BCSCs and BCSCs from BT549, MCF7, and clinical sample#5 cells. D, F Representative mammosphere images (D) and mammosphere-formation efficiencies (F) of BCSCs transfected with shPLA2G16-1, shPLA2G16-2 or treated with Giripladib (300 nM) for per generation, and their controls. E, G Representative mammosphere images (E) and mammosphere-formation efficiencies (G) of non-BCSCs transfected with LEV and LV-PLA2G16 lentivirus. n.d. not detected. H Ectopic PLA2G16 or control vector was transfected into lncROPM-silenced BCSCs; mammosphere formation abilities were evaluated by suspending culture. The representative mammosphere images (upper panel) and mammosphere-formation efficiencies (down panel) are shown. I Mammosphere formation abilities of non-BCSCs with ectopic lncROPM were assessed by suspending culture. And representative mammosphere images (upper panel) and mammosphere-formation efficiencies (down panel) are shown. n.d. not detected. Images were taken at the magnification of × 100 and scale bar was 200 µm. Data are presented as the mean ± SD; *p < 0.05, **p < 0.01