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. 2000 May;20(9):2959–2969. doi: 10.1128/mcb.20.9.2959-2969.2000

FIG. 10.

FIG. 10

Diagram illustrating the mechanisms involved in the regulation of Fli-1 mRNA translation by the conserved 5′ uORF. Step 1: Given the minimal twofold stimulation of the 51-kDa protein synthesis induced by the disruption of AUG −41 and GUG −37, we can estimate that at least half of the 40S subunits loaded at the 5′ end of Fli-1 mRNA initiate translation at upstream AUG −41 and GUG −37 codons. The remaining 40S subunits go on their 3′ progression by leaky scanning. Step 2: Translation initiation at AUG +1 is facilitated by the piling up of leaky-scanning 40S subunits against ribosomes terminating the translation of the two 5′ uORF at stop codons UAA +15 and UGA +35. Step 3: At least half of the translation initiated at AUG +100 is due to ribosomes which reinitiate after translation termination at stop codons UAA +15 and UGA +35, while the remaining translation is due to scanning 40S subunits having bypassed all upstream initiation codons.

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