FIG. 4.
Identification of the initiation codons involved in the synthesis of the 51- and 48-kDa FLI-1 proteins. (A) Schematic drawing of the 5′ ends of synthetic Fli-1 cDNA used to synthesize Fli-1 mRNA by in vitro transcription. Point mutations are underlined. (B) Equal amounts of each of these synthetic Fli-1 mRNA were used to program rabbit reticulocyte lysates in the presence of [35S]methionine. Translation products were then separated by SDS-PAGE, transferred to a nitrocellulose membrane, and revealed by autoradiography. (C) Western blot analysis of FLI-1 proteins synthesized in NIH 3T3 cells under transient expression of the transfected pCI Neo vector (Promega) carrying the indicated Fli-1 cDNA.