Figure 1. circ_0001806 is up-modulated in hepatocellular carcinoma (HCC). A, 15 circRNAs were differentially expressed in two microarray datasets (GSE94508, GSE97332) containing the circRNA expression profiles of HCC tissues and normal liver tissues. Heat maps showing the up-modulated circRNAs in HCC tissues compared to the matched non-tumor tissues GSE94508 (B) and GSE97332 (C). D, The levels of circ_0001806 were examined by qRT-PCR after treatment with RNase R. E, The subcellular distribution of circ_0001806 was analyzed by qRT-PCR after cellular RNA fractionation. GAPDH was used as the control for cytoplasm and U6 was used as the control for nucleus. F, The expression levels of circ_0001806 in 49 paired HCC tissues and matched adjacent normal tissues were examined by qRT-PCR. G, The expression levels of circ_0001806 in human HCC cell lines (Huh-7, HepG2, SMMC-7721, Bel-7402, and HL-7702) and the normal hepatic cell line (L02) were examined by qRT-PCR. Data are reported as means±SD. ***P<0.001 (ANOVA).