Figure 2. Knockdown of circ_0001806 inhibits multiplication, migration, and invasion of HCC cells. A, Three siRNAs targeting circ_0001806 (si-circ_0001806#1, si-circ_0001806#2, and si-circ_0001806#3) were transfected into Huh-7 and HepG2 cells and the expression level of circ_0001806 was detected by qRT-PCR. B, CCK-8 method was used to detect the multiplication of Huh-7 and HepG2 cells after transfection. C and D, Transwell experiment was employed to detect the migration and invasion of Huh-7 and HepG2 cells after transfection (scale bar 20 μm). Data are reported as means±SD. *P<0.05, **P<0.01, ***P<0.001; ns, not statistically significant (ANOVA and t-test).
