Figure 3. miR-193a-5p is the downstream target of circ_0001806. A, Bioinformatics analysis predicted that the sequence of miR-193a-5p matched the sequences of circ_0001806, and circ_0001806-WT (wild-type) and circ_0001806-MUT (mutant) luciferase reporter gene vectors were constructed. B, miR-193a-5p mimics (or miR control) were co-transfected with circ_0001806-WT (or circ_0001806-MUT) into HEK293T cells. After 48 h, the luciferase activity of the cells in each group was measured. C, The complex containing circ_0001806 and miR-193a-5p in Huh-7 and HepG2 cells was immunoprecipitated by anti-AGO2 using RIP assay. D, RNA pull-down assay was carried out to verify the interactions between circ_0001806 and miR-193a-5p. E, qRT-PCR was used to detect the expression of miR-193a-5p in Huh-7 and HepG2 cells after circ_0001806 knockdown. F, qRT-PCR was used to examine the expression levels of miR-193a-5p in 49 paired hepatocellular carcinoma (HCC) tissues and matched adjacent normal tissues. G, Pearson correlation analysis was utilized to evaluate the correlation between the expressions of circ_0001806 and miR-193a-5p in HCC tissues. Data are reported as means±SD. ***P<0.001 (t-test).