Figure 5. Circ_0001806 enhances the multiplication and invasion of hepatocellular carcinoma (HCC) cells through miR-193a-5p/MMP16 axis. Huh-7 and HepG2 cells were transfected with si-NC + anti-miR-Con, si-circ_0001806#1 + anti-miR-Con, or si-circ_0001806#1 + anti-miR-193a-5p. A, qRT-PCR was used to detect the expression of miR-193a-5p in Huh-7 and HepG2 cells. B, qRT-PCR was used to detect the expression of MMP16 mRNA in Huh-7 and HepG2 cells. C, Western blot was used to detect the expression of MMP16, MMP2, and MMP9 protein in Huh-7 and HepG2 cells. D, CCK-8 method was used to detect the multiplication of Huh-7 and HepG2 cells after transfection. The transwell experiment was employed to detect the migration and invasion of Huh-7 cells (E) and HepG2 cells (F) after transfection (scale bar 20 μm). Data are reported as means±SD. *P<0.05, **P<0.01, and ***P<0.001 (ANOVA).