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. 2021 Oct 15;15:772868. doi: 10.3389/fncel.2021.772868

FIGURE 5.

FIGURE 5

Impact of SE severity and duration on microgliosis. (A) Representative images of the hippocampus (CA1, CA1 close to the subiculum = CA1sub, CA3, DG), piriform cortex (PC) and amygdala (AMY). (B) Examples of reactive (M1-like, large cell body with retracted processes) and non-reactive (M2-like, small cell body with numerous fine branching patterns) IBA1 positive cells. M1-like microglia usually contained CD68 (yellow in the top panels). (C) Average number of IBA1 positive cells, (D) percent IBA1 positive cells colocalized with CD68, (E) percent IBA1 positive cells with reactive (M1)-like morphology (mixed measures ANOVA, n = 5-9). (F,G) Pooled data from all the hippocampal regions for the numbers of IBA1 positive cells (F) and IBA1 positive cells with reactive morphology (G, ANOVA, n = 5-8). (H,I) Western blotting for hippocampal lysates for IBA1 (ANOVA, n = 4-6). (J,K) Pooled data from the amygdala/piriform cortex for number of IBA1 positive cells (J) and IBA1 positive cells with reactive morphology (ANOVA, n = 5-8). (L,M) Western blotting for PC/AMY lysates for IBA1 (ANOVA, n = 4-6). Error bars indicate standard error of the mean. Scale, 100 μm (A) and 5 μm (B). *p < 0.05 compared to control. All graphs- mixed effects ANOVA, n = 5-12. Area counted = 0.14 mm2. Atlas Image credit: Allen Institute, United States.