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. 2021 Oct 15;15:757482. doi: 10.3389/fncel.2021.757482

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Copyright © 2021 Van Hoecke, Van Cauwenberghe, Dominko, Van Imschoot, Van Wonterghem, Castelein, Xie, Claeys, Vandendriessche, Kremer, Borghgraef, De Rycke, Hecimovic and Vandenbroucke.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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NPC1^–/– mice show a huge increase in autophagosomes in the choroid plexus epithelial cells. (A) Representative transmission electron microscopy (TEM) images of choroid plexus epithelial cells of the fourth ventricle of NPC1^+/+ and NPC1^–/– mice (7 and 9 weeks old). Scale bar represents 10 μm. The graph represents the amount of autophagosomes per cell section in 7 and 9 weeks old NPC1^+/+ (black) and NPC1^–/– (white) mice (n = 3). (B) Representative serial block face scanning electron microscope (SBF-SEM) snapshot of Supplementary Video 1 of a choroid plexus epithelial cell of the fourth ventricle of a 9 weeks old NPC1^–/– mouse. The autophagosomes are traced on the different sections and visualized in blue (n = 1). (C) Representative focused ion beam scanning electron microscopy (FIB-SEM) snapshot of Supplementary Video 2 of a choroid plexus epithelial cell of the fourth ventricle of a 9 weeks old NPC1^–/– mouse. The autophagosomes are traced on the different sections and visualized in blue (n = 1). (D) Representative images of LAMP1 staining (green) and VPS35 (red) of the choroid plexus of the fourth ventricle of NPC1^+/+ and NPC1^–/– mice of 7 and 9 weeks old. Cell nuclei are counterstained with Hoechst (blue) (n = 4–5). Scale bar represents 25 μm.